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superscript first strand synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa superscript first strand synthesis kit
    Superscript First Strand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rt+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pmc12869259-79-24-28
    Average 99 stars, based on 14861 article reviews
    superscript first strand synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Sulforaphane alleviates high fat diet-induced insulin resistance via AMPK/Nrf2/GPx4 axis.
    Article Snippet: .. First, Trizol reagent (Invitrogen) was used to isolate the RNA from liver tissues or HepG2 cells, and the isolated RNA was then used to synthesize the cDNA using a mix of oligo (dT) or random primers with the Superscript RT Kit (Takara Biomedical Technology Co. LTD) according to the manufacturer’s instructions. .. Quantitative real-time PCR was performed using a Roche LightCycler 480 PCR system and SYBR green fluorescence. β-actin or RP49 was used as the reference gene.

    Reverse Transcription:

    Article Title: Systematic re-analysis strategy of serum indices identifies alkaline phosphatase as a potential predictive factor for cervical cancer
    Article Snippet: Total RNA was isolated from cervical tissues using RNAiso Plus (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol. .. Total RNA was reverse transcribed into cDNA at 37°C for 15 min followed by 85°C for 5 sec using a Superscript RT kit (Takara Biotechnology Co., Ltd.) and amplified using the SYBR Premix Ex Tag TM kit (cat. no. RR420B; Takara Biotechnology Co., Ltd.), under the following conditions: Pre-denaturation at 95°C for 30 sec; 40 cycles of denaturation at 95°C for 3 sec, annealing at 60°C for 30 sec and extension at 72°C for 60 sec. ..

    Article Title: Exploration of the breast ductal carcinoma in situ signature and its prognostic implications
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Takara). .. The SuperScript RT kit (Takara) was utilized for reverse transcription. .. The SYBR Green PCR package (Takara) was used for the RT‐qPCR assay.

    Article Title: Lin28A and androgen receptor expression in ER-/Her2+ breast cancer.
    Article Snippet: The aim of this study was to examine the expression of Lin28A and androgen receptor (AR) in ER-/ Her2? breast cancer, and to research the association of Lin28A and AR co-expression status with patients’ prognosis.. The expression of Lin28A and AR in formalin-fixed and paraffin-embedded surgical sections from 305 patients with ER-/Her2? breast cancer was analyzed by immunohistochemistry, and the co-expression patterns in breast cancer cells were investigated by immunofluorescent staining.. The impact of the expression of Lin28A and AR in prognosis was also assessed by the Kaplan–Meier, univariate, and multivariate logistic regression models.

    Article Title: Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia-reperfusion
    Article Snippet: Guided by the manufacturer's instructions, total RNA was extracted using the TRIzol reagent (Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. Following measurement of RNA concentration, reverse transcription was performed using a SuperScript RT kit (Takara Biotechnology Co., Ltd., Dalian, China) under the following conditions: 37°C for 15 min, 85°C for 5 sec, and then maintained at 4°C. ..

    Article Title: Exploration of Core Genes Signature and Prognostic Implication for Ductal Carcinoma In Situ of the Breast
    Article Snippet: RNA extraction and reverse transcription quantitative polymerase chain reaction (RT-qPCR) Trizol reagent (Takara, Japan) was used to extract total RNA from cultured cells. .. For reverse transcription, the SuperScript RT kit (Takara, Japan) was used. .. The SYBR Green PCR package (Takara, Japan) was used for the RTqPCR assay.

    Amplification:

    Article Title: Systematic re-analysis strategy of serum indices identifies alkaline phosphatase as a potential predictive factor for cervical cancer
    Article Snippet: Total RNA was isolated from cervical tissues using RNAiso Plus (Takara Biotechnology Co., Ltd., Dalian, China) according to the manufacturer's protocol. .. Total RNA was reverse transcribed into cDNA at 37°C for 15 min followed by 85°C for 5 sec using a Superscript RT kit (Takara Biotechnology Co., Ltd.) and amplified using the SYBR Premix Ex Tag TM kit (cat. no. RR420B; Takara Biotechnology Co., Ltd.), under the following conditions: Pre-denaturation at 95°C for 30 sec; 40 cycles of denaturation at 95°C for 3 sec, annealing at 60°C for 30 sec and extension at 72°C for 60 sec. ..

    Article Title: Lin28A and androgen receptor expression in ER-/Her2+ breast cancer.
    Article Snippet: The aim of this study was to examine the expression of Lin28A and androgen receptor (AR) in ER-/ Her2? breast cancer, and to research the association of Lin28A and AR co-expression status with patients’ prognosis.. The expression of Lin28A and AR in formalin-fixed and paraffin-embedded surgical sections from 305 patients with ER-/Her2? breast cancer was analyzed by immunohistochemistry, and the co-expression patterns in breast cancer cells were investigated by immunofluorescent staining.. The impact of the expression of Lin28A and AR in prognosis was also assessed by the Kaplan–Meier, univariate, and multivariate logistic regression models.

    Polymerase Chain Reaction:

    Article Title: Lin28A and androgen receptor expression in ER-/Her2+ breast cancer.
    Article Snippet: The aim of this study was to examine the expression of Lin28A and androgen receptor (AR) in ER-/ Her2? breast cancer, and to research the association of Lin28A and AR co-expression status with patients’ prognosis.. The expression of Lin28A and AR in formalin-fixed and paraffin-embedded surgical sections from 305 patients with ER-/Her2? breast cancer was analyzed by immunohistochemistry, and the co-expression patterns in breast cancer cells were investigated by immunofluorescent staining.. The impact of the expression of Lin28A and AR in prognosis was also assessed by the Kaplan–Meier, univariate, and multivariate logistic regression models.

    Concentration Assay:

    Article Title: Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia-reperfusion
    Article Snippet: Guided by the manufacturer's instructions, total RNA was extracted using the TRIzol reagent (Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. Following measurement of RNA concentration, reverse transcription was performed using a SuperScript RT kit (Takara Biotechnology Co., Ltd., Dalian, China) under the following conditions: 37°C for 15 min, 85°C for 5 sec, and then maintained at 4°C. ..



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